The MicroCal VP-ITC is a powerful and sensitive, easy-to-use isothermal titration calorimeter that offers the ability to investigate any biomolecular interaction. Its fast, convenient label-free in solution analysis delivers the information needed to characterize the molecular interactions of proteins, antibodies, nucleic acids and other. MicroCalc, a J2ME spreadsheet (midlet), for java enabled handsets, phones, PDA. The MicroCal PEAQ-DSC system provides highly sensitive, easy-to-use microcalorimetry that helps reduce the time and cost associated with stability testing and comparability analysis. The MicroCal PEAQ-DSC is a manual instrument with a cleaning device, which can be. Older video editing software for mac 10.7.5. Package to MicroCal Software. You may also register at any time by contacting the Customer Support Department at MicroCal Software. Starting Origin To start Origin, double-click on the Origin 5.0 program icon on the Desk Top. Alternatively, click Start, then select Programs. Point to the MicroCal Origin50 folder and select the MicroCal Inc. The software was first published for the public in 1992 by Microcal Software, which later was renamed to OriginLab Corporation, located in Northampton, Massachusetts. Release history. 2020/4/30 Origin 2020b. Mini toolbar for worksheet & matrix, data connector navigator panel, browser graphs. Worksheet cells no longer showing ####.
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The MicroCal VP-ITC (Isothermal Titration Calorimeter) unit directly measures heat evolved or absorbed in liquid samples as a result of mixing precise amounts of reactants. A spinning syringe is utilized for injecting and mixing of reactants. The user inputs the experimental parameters (temperature, number of injections, injection volumes) and the computer carries out the experiment. Origin® software is then used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (KD), enthalpy (ΔH) and entropy (ΔS).
To avoid damage or contamination, researchers must provide their own Hamilton 81414 syringe for loading sample solutions. The syringe can be purchased through VWR via CyBuy or directly from Hamilton. The instrument must be reserved in advance, and various rules apply. Reserve time using iLab. Reservations do not require approval.
The instrument is located in 1238 Hach Hall. Enquiries should be directed to Sarah Cady. Phone 515-294-5975 (office). Lg slim portable dvd writer driver mac. NOTE: The Microcal ITC is now serviced and supported by the Malvern company. The weblink is: http://www.malvern.com/en/products/measurement-type/microcalorimetry/default.aspx.
Microcal Software
FY'18 Rates
The instrument is to be used by students and other researchers. Significant training is required, including sucessful completion of several tutorials and lessons before you will be authorized to use the instrument. Staff will NOT provide methods development assistance for this instrument, but will direct you to internet-based ITC resources. The hourly rates are listed below. NOTE: There is a minimum charge of $10 for each use of the instrument.
User Category | Instrument Charge | Staff Assistance |
---|---|---|
ISU researchers | $2.70 | $30 |
Non-ISU academic researchers | $4.05 | $45 |
Non-academic users | $8.10 | $90 |
Microcal VPITC Resources
- Origin 7 Data Processing Lessons
- Lesson 1: Routine ITC Data Analysis and Fitting
- Lesson 2: Setting Baseline and Integration Range
- Lesson 3: Deleting Bad Data
- Lesson 4: Analyzing Multiple Runs and Subtracting Reference
- Lesson 5: ITC Data Handling
- Lesson 6: Modifying Templates
- Lesson 7: Advanced Curve Fitting
- Lesson 8: Autosampler Data (Optional Accessory)
- Lesson 9: Other Useful Details
- Appendix: Equations Used for Fitting ITC Data
- VPITC Users Manual (factory)
Microcal Software
MicroCal PEAQ-DSC software 6 www.malvern.com 21 CFR Part 11 and Annex 11 Compliance software: DSC for the regulated environment The all-new MicroCal PEAQ-DSC software is available with an optional 21 CFR Part 11 compliance module. This makes both MicroCal PEAQ-DSC systems perfect for QC-type work, including root cause analysis and in-process. Etv bangla serial sadhak bamakhyapa songs download. Video driver update. Pentium e5400. Origin (digital distribution software), operated by EA (Electronic Arts) Origin (data analysis software), scientific graphing and data analysis software developed by OriginLab Corp; SGI Origin 200, a series of entry-level MIPS-based server computers made by Silicon Graphics.
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Differential Scanning Calorimetry (DSC) is used to measure the thermal transitions of DNA, proteins or peptides as they are heated or cooled. This is achieved by comparing the amount of energy required to heat a sample to the amount of energy required to heat the buffer of that sample (Δcp) at constant pressure. There are two cells located on top of the instrument termed sample (S) and reference (R) (Figure 1). Figure 1 The user should load 700 uL of buffer into both the sample and the reference cell and use a specially designed syringe to equal the level of the buffers between the two cells. Generally a program is set up and run overnight with both cells containing buffer to get a good baseline. The next morning the buffer in the sample cell will be replaced with your sample and the program will continue. After recording data, analysis can be performed in Origin software, which is provided with the instrument (Figure 2).
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Figure 2 It is important to note that in order to obtain consistent DSC data, it is advised that the user become familiar with the technique of loading the sample and reference cells. The instrument is very sensitive to any differences in the cells which may include any air in either cell and differences in the volumes loaded. These differences will lead to unusable data and users should become familiar with loading the cells using water until reproducible data is obtained.